Chitin content and chitin synthase activity as indicators of the growth of three different anaerobic rumen fungi
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چکیده
منابع مشابه
Chitin synthase homologs in three ectomycorrhizal truffles.
Degenerate PCR primers were used to amplify a conserved gene portion coding chitin synthase from genomic DNA of six species of ectomycorrhizal truffles. DNA was extracted from both hypogeous fruitbodies and in vitro growing mycelium of Tuber borchii. A single fragment of about 600 bp was amplified for each species. The amplification products from Tuber magnatum, T. borchii and T. ferrugineum we...
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Chs4p (Cal2/Csd4/Skt5) was identified as a protein factor physically interacting with Chs3p, the catalytic subunit of chitin synthase III (CSIII), and is indispensable for its enzymatic activity in vivo. Chs4p contains a putative farnesyl attachment site at the C-terminal end (CVIM motif) conserved in Chs4p of Saccharomyces cerevisiae and other fungi. Several previous reports questioned the rol...
متن کاملThe properties and localization of Saprolegnia monoica chitin synthase differ from those of other fungi.
The presence of non-fibrillar alpha-chitin in cellulosic fungi (class Oomycetes) poses intriguing questions as to its role, subcellular localization and evolutionary significance. Previous studies reported on the similarity of chitin synthase from Saprolegnia monoica with that of other fungi. The present work describes important dissimilarities. There was no evidence that the chitin synthase of...
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چکیده ندارد.
15 صفحه اولNucleotide sequence variation of chitin synthase genes among ectomycorrhizal fungi and its potential use in taxonomy.
DNA sequences of single-copy genes coding for chitin synthases (UDP-N-acetyl-D-glucosamine:chitin 4-beta-N-acetylglucosaminyltransferase; EC 2.4.1.16) were used to characterize ectomycorrhizal fungi. Degenerate primers deduced from short, completely conserved amino acid stretches flanking a region of about 200 amino acids of zymogenic chitin synthases allowed the amplification of DNA fragments ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1991
ISSN: 0378-1097
DOI: 10.1016/0378-1097(91)90216-w